Meal-uptake-dependent active microbiota and association with health and disease. A – Graphical representation of the genera distribution according to 16S rRNA assignation of metatranscriptomic reads (obtained by pyrosequencng of total cDNA) based on RDP classifier. Relative frequency for most predominant active genera is shown in dental plaque samples obtained before and after a carbohydrate-rich meal. B – Health- and disease-associated genera in the above metatranscriptome, as inferred from Linear Discrimination Analysis (LDA) performed for dimensional class comparisons. The data were generated by using LEfSe test, available in the Galaxy Web Server toolkit. NoCa = individuals with no caries; Ca = individuals with caries.