Analysis of the autoradiograms for changes in reliably detected de novo synthesized proteins. Three autoradiograms for each condition from independent experiments were analysed with the Delta 2D software. (A) The results from three gels were fused and spots color-coded: N = blue, N→H = orange, L = green, L→H = red. (B) A heat map was automatically constructed as described above based on the set of 129 reliably detected changes that were classified as significant with one way ANOVA (p ≤ 0.01). The lanes of the three identical conditions were placed next to each other. The four clusters were categorized according to the automatically generated cluster tree depicted on the left hand side. (C) Venn diagram of the significantly up-regulated spot intensities representing the overlaps among treatments.