The structure of orf288 and phylogenetic tree based on conserved orf288 genes in mitochondrial genomes. (A) The hau CMS-associated gene orf288 was located downstream of atp6. As for orf263 in Brassica tournefortii mitochondrial genome, the nucleotide identities (%) of the mitochondrial genome-derived fragments to the chimeric gene orf288 were shown. (B) Phylogenetic analysis of the CMS-associated gene orf288 in Brassicaceae. Orf263 was associated with cytoplasmic male sterility in Brassica tournefortii, Multiple sequence alignment used to construct the phylogenetic trees orf286, orf293 and orf305 shows they were separated in terms of sequence homology from the mitochondrial genomes of B. napus, B. juncea and R. sativus. Bootstrap values are shown at the nodes and the bar indicates the rate of nucleotide substitution per site.