Deep mRNA sequencing reveals stage-specific transcriptome alterations during microsclerotia development in the smoke tree vascular wilt pathogen, Verticillium dahliae

Table 1 RNA-Seq statistics

Sample	Insert size (bp)^a	Total reads^b	Mapped reads^c	Perfect match reads^d	Unmapped reads^e	Reads mapped to genes^f
CO	193.341 bp	27056079	23660245 (87.45%^g)	19767391 (73.06%^g)	3395834 (12.55%^g)	15750739 (58.22%^g/66.57%^h)
GC	195.521 bp	27056223	23268435 (86.00%)	19630309 (72.55%)	3787788 (14.00%)	15904017 (58.78%/68.35%)
MS1	188.009 bp	26190923	22036154 (84.14%)	18611864 (71.06%)	4154769 (15.86%)	14344871 (54.77%/65.10%)
MS2	194.213 bp	25596902	21727696 (84.88%)	18413763 (71.94%)	3869206 (15.12%)	14297493 (55.86%/65.80%)
MS3	195.993 bp	26410805	22551758 (85.39%)	19100267 (72.32%)	3859047 (14.61%)	14932675 (56.54%/66.22%)
MS4	190.443 bp	26190969	22745470 (86.84%)	19246089 (73.48%)	3445499 (13.16%)	14879393 (56.81%/65.42%)
Total		158501901	135989758 (85.80%)	114769683 (72.41%)	22512143 (14.20%)	90109188 (56.85%/66.26%)

^aThe length of fragments used for sequencing.
^bThe number of reads generated from sequencing after filtering low quality reads (Q ≤ 5).
^cThe number of reads mapped to the reference genome within 2 bp mismatch.
^dThe number of reads mapped to the reference genome with no mismatch.
^eThe number of reads that could not be mapped to the reference genome within 2 bp mismatch
^fThe number of reads mapped to the annotated genes within 2 bp mismatch.
^gThe percentages of reads account for the total reads.
^hThe percentages of reads account for the mapped reads.

ISSN: 1471-2164