In planta expression analysis for selected S . sclerotiorum effector candidates on four different hosts. (a) Transcriptional profiles of 16 S. sclerotiorum effector candidate genes. Overrepresented (yellow) and underrepresented transcripts (blue) in planta are shown as log2-fold changes relative to expression in vitro, normalized using Actin expression. Hierarchical clustering based on Pearson correlation coefficients delimited five clusters. The SS1G_11173 ubiquitin 16 gene was used as a non-induced control. (b) Sequential transcriptional activation of effector gene candidates during the infection of N. benthamiana (left) and A. thaliana Sha. accession (right). (c) In planta expression pattern of candidate effector genes showing host-independent expression (SS1G_06213, left) and host-dependent expression (SS1G_08858, right). (d) Differential expression patterns of two candidate effector genes on susceptible (Sha.) and resistant (Rub.) A. thaliana accessions. Relative gene expression shown as log2-fold changes relative to expression in vitro, normalized using Actin expression. Error bars show standard deviation calculated from two technical replicates on each of three independent biological experiments. Rub., Rubezhnoe; Sha., Shahdara.