Secretome analysis reveals effector candidates associated with broad host range necrotrophy in the fungal plant pathogen Sclerotinia sclerotiorum

Table 2 List of five S . sclerotiorum effector candidates selected based on Ka/Ks > 1 in pairwise comparisons with their B . cinerea orthologs

Protein ID	Annotation	PFAM domains	Length	Ka/Ks vs BC1T	KaKs vs BcT4	Ka/Ks > 1 sites	Prob
SS1G_04551	Pectinesterase A	Pectinesterase (PF01095.14)	308	2	2	max Ka/Ks = 0.93	NA
SS1G_07158	-	DUF1374 (PF07118)	328	6	ND	max Ka/Ks = 0.4	NA
SS1G_07749	Xylanase	Glycoside hydrolase family 11 (PF00457.12)	200	4	4	R36, L43, D74, Q76, S128, N174, S200	0.08224
SS1G_10165	Pectinesterase	Pectinesterase (PF01095.14)	310	2	0,061669	P22, K31, T34, A36, S54, A63, S85, S86, G88, S89, Q95, A118, D146, I165, F191, D208, P211, S212, T213, L218, 226I, 230A, 233S, 236A, 237G, 238 T, S246, V253, M258, S259, N260, 261 V, N263, V269, S274, P275, N276, 278Q, H285, A286, A290, H301, S302, P306, S310, N316, K318, S319, S324	0.00038
SS1G_10617	Glycoside hydrolase family 15 protein	Glycoside hydrolase family 15 (PF00723.16); Carbohydrate-binding module family 20 (PF00686.14); Carbohydrate-binding module family 25 (PF03423.8)	628	2	0,078172	N53, R56, M81, S91, N128, S313, S361, Q383, Q409, S416, N514, Y526, F537, V549, K586, V589, S605, S617, Q639	0.2987

Site specific Ka/Ks ratio estimated using Bayesian inference based on the M8 model of Yang et al., 2000. Only sites with Ka/Ks > 1 in the mature protein sequence are reported. P-value for positive selection estimated using a likelihood ratio test based on the comparison of twice the log likelihood difference obtained with the M8 and M8a null model with a chi-square probability table of degree of freedom 1. BC1T, Botrytis cinerea B.05 genome; BcT4, Botrytis cinerea T4 genome; ND, not determined.

ISSN: 1471-2164