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Table 2 Updated annotation of glycosyltransferases predicted in the genome of Lactobacillus rhamnosus GG

From: A network-based approach to identify substrate classes of bacterial glycosyltransferases

Locus tag Current annotation Proposed annotation HMM Evidence Reference
LGG_00279 welA; dTDP-rhamnosyl transferase rfbF wclA; glycosyltransferase (putative cell wall polysaccharide biosynthesis) Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_00280 welB; alpha-L-Rha alpha-1,3-L-rhamnosyltransferase wclB; glycosyltransferase (putative cell wall polysaccharide biosynthesis) Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_00281 welC; alpha-L-Rha alpha-1,3-L-rhamnosyltransferase wclC; glycosyltransferase (putative cell wall polysaccharide biosynthesis) Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_00283* eps2; hypothetical protein wclD; putative glycosyltransferase (putative cell wall polysaccharide biosynthesis) UDP-Glycosyltransferase - -
LGG_00295 Glycosyltransferase, group 2 Putative glycosyltransferase Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_00348 yohJ; lipopolysaccharide biosynthesis protein Putative glycosyltransferase UDP-Glycosyltransferase Conservation Kankainen et al., 2009 [44]
LGG_00349 yohH; polyglycerol-phosphate alpha-glucosyltransferase Putative glycosyltransferase UDP-Glycosyltransferase Conservation Kankainen et al., 2009 [44]
LGG_00645 Glycosyltransferase, group 2 Putative glycosyltransferase Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_00695 gtrB; glycosyltransferase, group 2 Putative glycosyltransferase Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_00794 pbp1B; penicillin-binding protein 1B pbpb1B; putative glycosyltransferase, penicillin-binding protein 1B (peptidoglycan biosynthesis) Pfam/CAZy Conservation Kankainen et al., 2009 [44]
LGG_00825 rfaG; glycosyltransferase, group 1 Putative glycosyltransferase UDP-Glycosyltransferase Conservation Kankainen et al., 2009 [44]
LGG_00826 cpoA; glycosyltransferase, group 1 Putative glycosyltransferase UDP-Glycosyltransferase Conservation Kankainen et al., 2009 [44]
LGG_00928* yvcK; transporter Putative glycosyltransferase Rossmann-fold domains - -
LGG_00985* Integral membrane protein Putative glycosyltransferase Pfam/CAZy - -
LGG_00998 arbX; lipopolysaccharide biosynthesis glycosyltransferase Putative glycosyltransferase Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_00999 arbY; lipopolysaccharide biosynthesis glycosyltransferase Putative glycosyltransferase Rossmann-fold domains Conservation Kankainen et al., 2009 [44]
LGG_01057 Glycosyltransferase, group 2 Putative glycosyltransferase Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_01062# galU; UTP-glucose-1-phosphate uridylyltransferase UTP-glucose-1-phosphate uridylyltransferase Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_01069 gtrB; glycosyltransferase, group 2 Putative glycosyltransferase Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_01147 Glycosyltransferase, group 1 Putative glycosyltransferase Rossmann-fold domains Conservation Kankainen et al., 2009 [44]
LGG_01195* metQ; ABC transporter ABC transporter, putative bifunctional glycosyltransferase Pfam - -
LGG_01283 murG; undecaprenyldiphospho-muramoylpentapeptide beta-N- acetylglucosaminyltransferase murG; undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (peptidoglycan biosynthesis) UDP-Glycosyltransferase Conservation Mengin-Lecreulx et al., 1991 [26]; Kankainen et al., 2009 [44]
LGG_01412* trmFO; tRNA uracil-5-methyltransferase tRNA uracil −5-methyltransferase, putative bifunctional glycosyltransferase Rossmann-fold domains - -
LGG_01487 pbp1A; penicillin-binding protein 1A pbp1A; putative glycosyltransferase, penicillin-binding protein 1A (peptidoglycan biosynthesis) Pfam/CAZy Conservation Kankainen et al., 2009 [44]
LGG_01538 Phage-related glycosyltransferase Putative glycosyltransferase Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_01586 yohH; glycosyltransferase, group 1 Putative glycosyltransferase UDP-Glycosyltransferase Conservation Kankainen et al., 2009 [44]
LGG_01587 yohJ; glycosyltransferase, group 1 Putative glycosyltransferase Rossmann-fold domains Conservation Kankainen et al., 2009 [44]
LGG_01783 pbp2A; membrane carboxypeptidase, penicillin-binding protein 2A pbp2A; bifunctional membrane carboxypeptidase, putative glycosyltransferase, penicillin-binding protein 2A (peptidoglycan biosynthesis) Pfam/CAZy Conservation Di Guilmi et al., 2003 [17]; Kankainen et al., 2009 [44]
LGG_01991* UDP-N-acetylglucosamine 2-epimerase Epimerase, putative bifunctional glycosyltransferase UDP-Glycosyltransferase - -
LGG_01992* UDP-N-acetylglucosamine 2-epimerase Epimerase, putative bifunctional glycosyltransferase Sugar transferase - -
LGG_01999 rmlA; glucose-1-phosphate thymidylyltransferase rmlA; glucose-1-phosphate thymidylyltransferase Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_02004 eps3; sugar or lipopolysaccharide synthesis transferase Putative glycosyltransferase Pfam Conservation Kankainen et al., 2009 [44]
LGG_02023# glgP; glycogen starch alpha-glucan phosphorylase glgP, glycogen alpha-glucan phosphorylase UDP-Glycosyltransferase Conservation Kankainen et al., 2009 [44]
LGG_02024 glgA; glycogen synthase glgA; glycogen synthase (glycogen biosynthesis) UDP-Glycosyltransferase Conservation Kiel et al., 1994 [42]; Kankainen et al., 2009 [44]
LGG_02025# glgD; glucose-1-phosphate adenylyltransferase glgD; glucose-1-phosphate adenylyltransferase (glycogen biosynthesis) Sugar transferase Conservation Ballicora et al., 2003 [56]; Kankainen et al., 2009 [44]
LGG_02026# glgC; glucose-1-phosphate adenylyltransferase glgC; glucose-1-phosphate adenylyltransferase (glycogen biosynthesis) Sugar transferase Conservation Ballicora et al., 2003 [56]; Kankainen et al., 2009 [44]
LGG_02040$ rmlA1; glucose-1-phosphate thymidyl transferase rmlA1; glucose-1-phosphate thymidyl transferase Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_02042 rmlA2; glucose-1-phosphate thymidylyltransferase rmlA2; glucose-1-phosphate thymidylyltransferase Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_02043 welE; undecaprenyl-phosphate beta-glucosephosphotransferase welE; priming glycosyltransferase (galactose-rich EPS biosynthesis) Pfam Experimental validation Lebeer et al., 2009 [37]
LGG_02044 welF; glycosyltransferase, group 1 welF; putative glycosyltransferase (galactose-rich EPS biosynthesis) UDP-Glycosyltransferase Conservation Kankainen et al., 2009 [44]
LGG_02045 welG; glycosyltransferase, galactofuranosyltransferase welG; putative glycosyltransferase (galactose-rich EPS biosynthesis) UDP-Glycosyltransferase Conservation Kankainen et al., 2009 [44]
LGG_02046 welH; alpha-L-Rha alpha-1,3-L-rhamnosyltransferase welH; putative glycoysltransferase (galactose-rich EPS biosynthesis) Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_02047 welI; glycosyltransferase, group 1 welI; glycosyltransferase (galactose-rich EPS biosynthesis) UDP-Glycosyltransferase Experimental validation This work
LGG_02284 Glycosyltransferase, group 1 Putative glycosyltransferase UDP-Glycosyltransferase Conservation Kankainen et al., 2009 [44]
LGG_02285 yohH; glycosyltransferase, group 1 Putative glycosyltransferase UDP-Glycosyltransferase Conservation Kankainen et al., 2009 [44]
LGG_02347* Hypothetical protein Putative glycosyltransferase Pfam - -
LGG_02562# glmU; UDP-N-acetylglucosamine pyrophosphorylase UDP-N-acetylglucosamine pyrophosphorylase Sugar transferase Conservation Kankainen et al., 2009 [44]
LGG_02869 Glycosyltransferase, group 1 Putative glycosyltransferase UDP-Glycosyltransferase Conservation Kankainen et al., 2009 [44]
  1. Locus tag: gene identifier of the predicted GT. Genes for which a GT activity was predicted in this study that was not present in the current annotation are marked with a star (*). Potential false positive results are indicated with a hash (#). Current annotation: functional annotation as in current genome release of GenBank (NC_013198.1). Proposed annotation: new annotation proposed based on the results of our analysis. HMM: description of the Hidden Markov Model (HMM) with which the indicated GT was identified. Evidence: Level of evidence for the GT activity. Conservation: shows significant sequence conservation with an experimentally validated GT in a closely related species. Experimental validation: the GT activity has been experimentally validated in Lactobacillus rhamnosus GG. Reference: reference to the publication(s) supporting the evidence.