Comparison of non-mutagenized parent strains: 2F-1-YFP2, Blader RH-ΔHGXPRT (B-RH) and Gubbels RH-ΔHGXPRT (G-RH). (A) Venn diagram of shared and unique SNVs between the three strains and the GT1 reference genome. (B) The incidence of various mutations causing changes in amino acid coding. For 2F-1-YFP2, B-RH, and G-RH the unique SNVs vs GT1 are shown (these correspond to the 54, 66, and 19 SNVs in panel A, respectively). “RH-srd.” refers to all SNVs shared between B-RH and G-RH and “all-srd.” refers to the SNVs shared between all three lines (these correspond to the and 85 and 984 SNVs in panel A, respectively) Syn.: synonymous; non-syn: non-synonymous. * 50 non-coding SNVs map to the apicoplast genome. (C-E) The incidence of the various base pair changes (SNVs) in the mutants and groups as indicated. Note that we used the n-F-P2 reads instead of 2F-1-YFP2 reads in this analysis since the quality is much higher (Figure 2); ENU specific n-F-P2 mutations were removed from the comparative analysis.