Maximum likelihood tree of aggregrate spider glue 2 (AgSG2) and homologous unique assembled transcripts (UATs). The trees were estimated from alignments of complete protein sequences (A) and domain-specific sequences (B). Trees are midpoint rooted. Trinity-defined isoforms of a single component are shown in blue while the published Nephila clavipes (Nc) and published Latrodectus hesperus (Lh) sequences are shown in purple and orange, respectively. All bootstrap values greater than 50 are shown above branches. Expression rank and FPKM (fragments per thousand base pairs per million mapped fragments) are shown on the right. Rank is based on FPKM in the silk glands out of all UATs expressed in silk glands.