Gene expression profiling of 49 human tumor xenografts from in vitro culture through multiple in vivo passages - strategies for data mining in support of therapeutic studies

Hollingshead, Melinda G; Stockwin, Luke H; Alcoser, Sergio Y; Newton, Dianne L; Orsburn, Benjamin C; Bonomi, Carrie A; Borgel, Suzanne D; Divelbiss, Raymond; Dougherty, Kelly M; Hager, Elizabeth J; Holbeck, Susan L; Kaur, Gurmeet; Kimmel, David J; Kunkel, Mark W; Millione, Angelena; Mullendore, Michael E; Stotler, Howard; Collins, Jerry

doi:10.1186/1471-2164-15-393

Table 1 Tumor cell lines, host mice, protocol variances

From: Gene expression profiling of 49 human tumor xenografts from in vitro culture through multiple in vivo passages - strategies for data mining in support of therapeutic studies

Tumor type^a	Tumor name	Histology	Mouse strain	Comments
Breast	MCF7^b	Adenocarcinoma	nu/nu NCr	2 samples P0, 4 samples P1
	MDA-MB-231 T	Adenocarcinoma, from mouse	nu/nu NCr	2 samples P0
Cervical	HeLa-Luc	Adenocarcinoma	nu/nu NCr	2 samples P0
CNS	SF-268^c	Anaplastic Astrocytoma	NOD.SCID/NCr	2 samples P0, P4 and P10 are mouse
	SF-539^c	Glioblastoma	NOD.SCID/NCr	2 samples P0, P4 and P10 are mouse
	U251	Glioblastoma	nu/nu NCr	2 samples P0
Colon	COLO 205	Adenocarcinoma	nu/nu NCr
	HCC-2998	Carcinoma	nu/nu NCr
	HCT-116	Carcinoma	nu/nu NCr
	HCT-15	Adenocarcinoma	nu/nu NCr
	HT-29	Adenocarcinoma, GR III	nu/nu NCr
	KM12	Adenocarcinoma	nu/nu NCr
	SW-620	Adenocarcinoma	nu/nu NCr
Gastric	GTL 16	Adenocarcinoma	nu/nu NCr	2 samples P0
Leukemia/	AS283	AIDs related Burkitts Lymphoma	SCID/NCr	2 samples P0
Lymphoma	CA46	B Lymphocyte Burkitts Lymphoma	SCID/NCr	2 samples P0
	CCRF-CEM	Acute Lymphoblastic Leukemia (T-ALL)	SCID/NCr
	HL-60(TB)	Promyelocytic Leukemia	SCID/NCr	3 samples P5 substituted for P4; 4 P0 samples
	K-562	Chronic Myelogenous Leukemia	SCID/NCr	5 samples P5 substituted for P4, 4 samples P10
	MOLT-4	Acute Lymphoblastic Leukemia	SCID/NCr
	SR	Large Cell, Immunoblastic	SCID/NCr
Liver	HuH-7	Differentiated Hepatocellular Carcinoma	nu/nu NCr	2 samples P0
Lung	A549	Adenocarcinoma	nu/nu NCr
	A549/Asc-1	Adenocarcinoma	nu/nu NCr	3 samples P4, 2 samples P0
	EKVX	Adenocarcinoma	SCID/NCr	2 samples P0
	HOP-62	Adenocarcinoma	nu/nu NCr	4 samples P1
	HOP-92	Large Cell, undifferentiated	nu/nu NCr
	NCI-H226	Squamous Carcinoma	nu/nu NCr	2 samples P1
	NCI-H23	Adenocarcinoma, NSCLC	nu/nu NCr	2 samples P0
	NCI-H460	Large Cell Carcinoma	nu/nu NCr
	NCI-H522	Adenocarcinoma	nu/nu NCr	2 samples P0
Melanoma	A375	Metastatic Malignant Melanoma	nu/nu NCr	2 samples P0
	COLO 829	Malignant Melanoma	nu/nu NCr	2 samples P0
	LOX IMVI	Malignant Amelanocytic Melanoma	nu/nu NCr	2 samples P0
	M14	Adenocarcinoma	nu/nu NCr	2 samples P0
	MALME-3 M	Malignant Melanoma	nu/nu NCr	2 samples P0, 4 samples P4
	MDA-MB-435	Adenocarcinoma	nu/nu NCr	2 samples P0, 3 samples P10
	MDA-N^d	HER2/ERB2 transfectant of MDA-MB-435	nu/nu NCr	2 samples P0
	UACC-62	Malignant Melanoma	nu/nu NCr
Ovarian	CP70	Carcinoma	nu/nu NCr	2 samples P0
	OVCAR-3	Adenocarcinoma	nu/nu NCr	2 samples P0
	OVCAR-5	Carcinoma	nu/nu NCr	2 samples P0
Pancreatic	AsPC-1	Adenocarcinoma	nu/nu NCr	2 samples P0
Prostate	PC-3	Adenocarcinoma	Male nu/nu NCr	2 samples P0
	PC-3/M^e	Metastatic PC-3 subline	Male nu/nu NCr
Renal	786-0	Adenocarcinoma	nu/nu NCr
	CAKI-1	Renal Cell Carcinoma	nu/nu NCr	2 samples P0
	RXF 393	Poorly Differentiated Hypernephroma	nu/nu NCr
	SN12C	Carcinoma	nu/nu NCr	2 samples P0

^aTumor cells derived from the 4th in vitro passage were innoculated subcutaneously into mice. Tumors were passaged 10 consecutive times in mice. At passages 1, 4, and 10 (P1, P4, P10, respectively), 5 tumors were harvested, cut into small pieces and flash frozen in liquid nitrogen. Samples were then processed as described in Methods. For comparative purposes, 3 samples of the in vitro cultivated cells at passage 4 (P0) were also prepared for microarray analysis.
^b3 mg/kg Q7D SC Estradiol.
^cMatrigel 18.1 mg/mL P1 only.
^dHER2/ERBB2 transfectant of MDA-MB-435.
^ePC3 subline isolated from liver metastasis in mice.

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