Comparison of miRNAs expression profiles from human PBMCs and serum. Human PBMCs (1×106 cells) and serum (300 μL) samples were extracted by Macherey-Nagel (MN) and Qiagen (Q) kits. A- Number of miRNAs detected from PBMCs RNA samples using TLDA cards. B- Correlation analysis from PBMCs samples. C- Bland-Altman analysis MN versus Q from PBMCs. D- Number of miRNAs detected from serum RNA samples using TLDA cards. E- Correlation analysis from serum samples. F- Bland-Altman analysis MN versus Q from serum. TLDA data were obtained from biological triplicate for each extraction kits. Analysis using mean Ct values of triplicate. Only miRNAs with Ct < 32 were considered for PBMCs. No Ct cut-off was applied for serum miRNAs.