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Table 1 Assessment of quantity and quality of RNA isolated

From: Comparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells

A   Macherey Nagel Qiagen Norgen
PBMC n 8 10 10
Nanodrop Total RNA: Mean quantity (ug/1×106 cells or ml serum) (SD) 1.03 (0.22) 0.97 (0.14) 1.93 (0.69)
Ratio OD (260 nm/280 nm) (SD) 2.04 (0.07) 1.75 (0.06) 1.91 (0.07)
Agilent Nano 6000 Chip Total RNA: Mean Quantity (ug/1×106 cells or ml serum) (SD) 1.19 (0.33) 0.96 (0.30) 0.98 (0.50)
RIN mean (SD) 8.84 (0.34) 8.73 (0.41) 7.33 (2.93)
B   Macherey Nagel Qiagen Norgen
Serum n 10 10 10
Nanodrop Total RNA: Mean quantity (ug/1×106 cells or ml serum) (SD) 0.91 (0.46) 0.89 (0.40) 0.99 (0.34)
  Ratio OD (260 nm/280 nm) (SD) 1.24 (0.11) 1.40 (0.13) 1.27 (0.52)
  1. The quantity and quality of RNA isolated from (A) PBMCs and (B) serum using three RNA extraction kits (Macherey-Nagel, Qiagen, Norgen) were evaluated by Nanodrop, Agilent nano 6000 (PBMCs only) and Agilent small RNA chips.