Comparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells

Table 1 Assessment of quantity and quality of RNA isolated

A		Macherey Nagel	Qiagen	Norgen
PBMC	n	8	10	10
Nanodrop	Total RNA: Mean quantity (ug/1×10⁶ cells or ml serum) (SD)	1.03 (0.22)	0.97 (0.14)	1.93 (0.69)
Nanodrop	Ratio OD (260 nm/280 nm) (SD)	2.04 (0.07)	1.75 (0.06)	1.91 (0.07)
Agilent Nano 6000 Chip	Total RNA: Mean Quantity (ug/1×10⁶ cells or ml serum) (SD)	1.19 (0.33)	0.96 (0.30)	0.98 (0.50)
Agilent Nano 6000 Chip	RIN mean (SD)	8.84 (0.34)	8.73 (0.41)	7.33 (2.93)
B		Macherey Nagel	Qiagen	Norgen
Serum	n	10	10	10
Nanodrop	Total RNA: Mean quantity (ug/1×10⁶ cells or ml serum) (SD)	0.91 (0.46)	0.89 (0.40)	0.99 (0.34)
	Ratio OD (260 nm/280 nm) (SD)	1.24 (0.11)	1.40 (0.13)	1.27 (0.52)

The quantity and quality of RNA isolated from (A) PBMCs and (B) serum using three RNA extraction kits (Macherey-Nagel, Qiagen, Norgen) were evaluated by Nanodrop, Agilent nano 6000 (PBMCs only) and Agilent small RNA chips.

ISSN: 1471-2164