Figure 5
From: Multiplex genomic structure variation mediated by TALEN and ssODN
Large chromosomal deletions using a pair of TALENs and ssODN. (A) Mosaic mutations induced by co-injection of TALEN-B3 and ssODN. Silkworms were reared on fresh mulberry leaves. (B) Images of germline mutations from the dorsal side (left panel) and the lateral side (right panel). The worm on the left in each panel is wild type silkworm. Silkworms were reared on artificial diet. The scale bar represents 1 cm. (C) Gel analysis of PCR amplifications using primers B2-F241 and B3-R315 from genomic DNA of 5 G1 mutant individuals (numbers 1 to 5). M represents the DNA ladders, the letters on the left indicate length of each band. (D) Gel analysis of PCR amplifications using genomic DNA of wild type (panel indicated as 1 to 3) and G2 mutant silkworm from NO. 4 G1 mutants (panel indicated as 4 to 6). Primer sets are shown on the top of each panel.