MicroRNA expression profiling of the fifth-instar posterior silk gland of Bombyx mori

BMC Genomics

Table 1 Data summary of sequenced small RNAs based on DSAP

	Q1	Q2	B1	B2	QB1	QB2	BQ1	BQ2	R1	J1
Total reads	18707473	18633295	18556476	18499427	17947940	16942708	17449851	17905942	10508573	12079631
Cleaned sequence tags	901684	674947	810182	622202	633421	617616	857114	617471	1162861	816340
Reads in cleaned sequence tags	10044506	10693830	10790311	9085018	7461204	8113977	9405884	9159736	8614699	9822808
% reliable reads ^#	53.69	57.39	58.15	49.11	41.57	47.89	53.90	51.15	81.98	81.32
Unique Sequence Clusters (USC)	901684	674947	810182	622202	633421	617616	857114	617471	1162861	816340
Matched ncRNA in Rfam	690	608	673	596	642	569	701	602	739	693
USC matched to Rfam	134580	97673	122849	105997	89704	92104	127959	101047	154898	145107
Reads matched to Rfam	2729256	2440852	2908825	2573860	1644409	2172466	2133486	2565135	1108916	1386447
% reads matched to Rfam	27.17	22.82	26.96	28.33	22.04	26.77	22.68	28	12.87	14.11
Matched miRNAs in miRBase	181	170	182	157	181	150	202	168	239	220
USC matched to miRBase	1411	1167	1216	927	1174	817	1643	1016	2540	2089
Reads matched to miRBase	406154	744285	540458	277369	486835	339576	544337	310026	1486269	1576622
% reads matched to miRNAs	4.04	6.96	5.01	3.05	6.52	4.19	5.79	3.38	17.25	16.05
USC Unmatched reads	765693	576107	686117	515278	542543	524695	727512	515408	1005423	669144

Note: #Percentage of reliable reads = (Number of reads in cleaned sequence tags/Number of total reads)*100.

ISSN: 1471-2164