Figure 4

Regulation by PhLP1, GNB1 and GNG1. Influence on gnb1 (A and B) and gna1 (C and D) transcript levels in response to light and influence of GNB1 on gna1 transcription (E). Transcript levels were measured by qRT-PCR from strains grown on Mandels-Andreotti minimal media with 1% (w/v) glycerol as carbon source. Strains were grown on Mandels-Andreotti minimal media with 1% (w/v) glycerol as carbon source for 24 hours in darkness (24DD) and then exposed to light for 15, 30, 60 or 120 minutes (minutes DL), respectively.