Expression of AGO2 mRNA, protein and effect of knocking down of AGO2 mRNA in the regulation of miRNAs expression and trophoblastic stem cell differentiation. A: Expression of AGO2 protein in the day-50 placentas from AI, NT and IVP derived pregnancies (A). Expression of AGO2 mRNAs (B) and protein (C) 48 hours after transfection of AGO2 siRNA (siAGO2), nontransfected control (None) and transfection of nonspecific scrambled siRNAs (siSCR) in the TBSs during their differentiation in vitro. D: Expression of selected candidate miRNAs in three groups of treated TBSs (siAGO2, none and siSCR). Images on the expression of AGO2 and the level of alkaline phosphatase activity (APA) in the same three groups of cells (E).