Figure 6From: Massive deregulation of miRNAs from nuclear reprogramming errors during trophoblast differentiation for placentogenesis in cloned pregnancyEffect of knocking down of AGO2 mRNA in the regulation of trophoblastic stem cell differentiation. Expression of AGO2 protein (green) 48 hours after transfection of AGO2 siRNA (siAGO2) and nontransfected control (None) in the TBSs during their differentiation in vitro. Trophoblastic giant cells, Glycogen cells and Spongiotrophoblast cells are indicated by red, blue and green arrowhead. White arrow and red circle indicates binucleate cells and endopolyploidy level identified by the blue nuclear stain (DAPI) in the images including inset. Scale bar: 100 μm (50 μm in the right column).Back to article page