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Figure 4 | BMC Genomics

Figure 4

From: RNA-seq analysis of the influence of anaerobiosis and FNR on Shigella flexneri

Figure 4

qRT-PCR verification of S. flexneri virulence plasmid genes repressed under anaerobic growth conditions and the role of FNR in the repression. Strand specific qRT-PCR analysis of S. flexneri M90T virulence plasmid genes mRNA levels shown to be repressed in RNA-seq analysis. Data were calculated as the n-fold difference relative to polA (2Ct, where ΔCt represents the difference in threshold cycle between the target and control genes). Results are shown in relation to the wild-type 2Ct levels under aerobic conditions (referred to as 1). Values greater than 1 indicate increased transcription under anaerobiosis, while lower than 1 indicate the opposite. Significant differences were detected with the wild-type strain 2Ct levels under aerobic and anaerobic conditions, or wild-type vs. Δfnr 2Ct levels under anaerobiosis were compared. P <0.01, **; n = 4; Mann–Whitney test. Error bars show Standard Deviation (SD).

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