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Table 2 Whole genome sequence analysis of B. cereus genomic and iMDA DNA

From: Improved Multiple Displacement Amplification (iMDA) and Ultraclean Reagents

 

Genomic-1

Genomic-2

iMDA-1 pg

Total MB

445

574

398

≥ 1X coverage

99.978%

99.979%

99.975%

≥ 5X coverage

99.941%

99.939%

99.898%

Average read length

224

213

212

Average coverage

82

105

64

Total reads

1989230

2696410

1879724

Mapped reads*

1988492

2695230

1879079

B. cereus**

99.80%

99.62%

99.64%

  1. *Mapped reads were identified with the Ibis Galaxy analysis.
  2. **Includes all reads that mapped to the B. cereus clade, (i.e., B. cereus, B. thuringiensis, and B. anthracis).