Figure 1
From: Methylation-capture and Next-Generation Sequencing of free circulating DNA from human plasma
Comparison of fcDNA isolation from plasma and serum. (A) PCR quantitation of fcDNA obtained from 200 μL plasma and serum samples. Bars represent the average concentration fold change in matched serum and plasma samples obtained from 3 separate donors ± SD. (B) Lane 1: fcDNA isolated from 4 mL serum; Lane 2: fcDNA isolated from 4 mL plasma; Lane 3: genomic DNA spiked into serum sample prior to fcDNA processing; Lane 4: genomic DNA spiked into plasma sample prior to fcDNA processing; Lane 5: genomic DNA. Arrow: high molecular weight genomic DNA; MWM: molecular weight marker.