Figure 2
From: Methylation-capture and Next-Generation Sequencing of free circulating DNA from human plasma
Contamination of fcDNA with genomic DNA during blood storage. Concentration change of DNA extracted from plasma (A) or serum (C) measured by PCR at 0 hr, 4 hr, 8 hr, 24 hr and 48 hr following blood collection. Each bar represents the average concentration fold change of triplicate (A) or duplicate (C) experiments ± SD. (B) fcDNA isolated from 4 mL plasma immediately (Lane 1), 8 hrs (Lane 2), 24 hrs (Lane 3) and 48 hrs (Lane 4) following blood collection. Lane 5: genomic DNA. (D) fcDNA isolated from 3 mL serum immediately (Lane 1), 4 hrs (Lane 2), 8 hrs (Lane 3), 24 hrs (Lane 4) and 48 hrs (Lane 5) following blood collection. Lane 6: genomic DNA. Arrow: High molecular weight genomic DNA; MWM: molecular weight marker.