Figure 5

Clonal bisulphite sequencing of fcDNA from sample 5. (A) Validation of 3 promoter regions found to be methylated in SSSI positive control (green) and in fcDNA (blue). (B) Validation of 3 promoter regions found to be methylated SSSI positive control (green) but not in fcDNA (blue). (C) Validation of 3 loci found to be unmethylated in SSSI positive control (green) but methylated in fcDNA (blue). Closed circles indicate methylated CpGs as determined by clonal bisulphite sequencing; open circles indicate unmethylated CpGs as determined by clonal bisulphite sequencing; grey arrows indicate transcriptional start sites; red rectangles indicate the regions sequenced for validation.