Table 1 Concentration of fcDNA in 5 control subjects and DNA quantitation at consecutive stages of sample processing
From: Methylation-capture and Next-Generation Sequencing of free circulating DNA from human plasma
Sample | Plasma fcDNA concentration (ng/mL) | MethylMiner input (ng) | MethylMiner recovery (ng) (%) | Illumina NGS* input for library generation (ng) | Amount of library generated (ng) |
|---|---|---|---|---|---|
1 | 6.9 | 48.7 | 5.04 (10.4) | 4 | 464 |
2 | 7.3 | 49.6 | 5.04 (10.2) | 4 | 245 |
3 | 7.8 | 49.5 | 5.73 (11.6) | 4 | 314 |
4 | 9.9 | 42.2 | 6.28 (14.9) | 4 | 39 |
5 | 10.7 | 43.2 | 5.32 (12.3) | 4 | 222 |