Methylation-capture and Next-Generation Sequencing of free circulating DNA from human plasma

BMC Genomics

Table 2 Descriptions of Next-Generation Sequencing read numbers

Sample	Total reads¹	Unaligned²	Multiple site aligned³	Single site aligned⁴	Unique⁵	% Unique
1	197,921,529	16,066,294	44,319,996	137,535,239	76,590,535	55.7
2	199,302,552	15,885,135	43,676,951	139,740,466	77,836,232	55.7
3	108,955,707	8,729,606	26,671,313	73,554,788	43,869,724	59.6
4	109,132,802	11,042,561	24,138,010	73,952,231	37,270,330	50.4
5	200,639,214	15,558,416	44,708,970	140,371,828	85,538,368	60.9

¹Total reads obtained.
²Reads which could not be aligned to a site within the human genome.
³Reads which could not be accurately mapped since they aligned to multiple sites within the human genome.
⁴Reads which aligned to a single site within the human genome.
⁵Unique reads which aligned to a single site within the human genome.
⁶Unique reads as a percentage of the reads which could be aligned at a single site within the genome.

ISSN: 1471-2164