Figure 3

Workflow for sequencing and analysis of miRNA changes. (A) Flowchart depicting miRNA sample preparation and sequencing. After extracting the total RNA from the samples, gel select Small RNA (18 ~ 30 nt), 5' RNA adapter ligation and gel purification, 3' RNA adapter ligation and gel purification, and RT-PCR and gel purification, the library products were ready for sequencing analysis via Illumina HiSeqTM 2000. (B) Flowchart depicting bioinformatic processing and analysis. After sequencing, raw reads were cleaned by removing low quality reads and short reads. Reads were profiled by mapping them to miRBase v. 20 and other sequence databases. (C) Summary (percent) of size distribution of small RNA sequences in the urinary samples analyzed.