Figure 4

Quantitative allele frequency measurement in DNA pools. Genotyping of parental strains (2029-C5 in brown and 1782-B1 in deep purple), and two segregating pools with low (LNR orange) and high (HNR in purple) nitrogen requirements. The 2029-C5 allele enrichment of the pools is indicated by deviations above 0 and 1782-B1 allele enrichment is indicated by deviations below 0. Regions with significant differences in allelic frequencies between the two pools are indicated by light green dots. RM11 probes were positioned on the S288C genome by Blast. Four regions displaying large differences between the two pools were chosen for further analysis: A1, A2, B and C.