Figure 1

Sequence-based Aac difference between T4S and control proteins for C-terminal 100-aa positions. (A) Single-residue composition difference. The different amino acids were listed along the horizontal axis while the length of bars represented the frequency of the corresponding amino acid. T4S and non-T4S proteins were represented in black and gray, respectively. Amino acid with significant different compositions between effectors and non-effectors were indicated with a star above the bar (Bonferroni-corrected Student’s t test and binomial test, p < 0.05). The logarithm of amino acid frequency ratio was also shown, with red representing preference and black representing depletion in effectors. (B) Continual and spanned bi-residues with statistically significant composition difference between effectors and non-effectors (Bonferroni-corrected Student’s t test and binomial test, p < 0.05). ‘Px’ represented ‘Position x’. ‘X’ represented any type of amino acid. The amino acid at the last position was in red if the corresponding bi-residue was preferred and in black if depleted in T4S sequences. (C) Distribution of motifs in T4S and non-T4S proteins.