Figure 2

Genome alignment and annotation for phages ϕA318 and ϕAs51 with the genomes from phages SP6 and K1E. (A) Genome alignment using query of ϕA318 against to phages ϕAs51, SP6 and K1E, (B) Alignment using query of SP6 against phages K1E, ϕA318, and ϕAs51. ϕAs51 map is highly similar to ϕA318 with 5 single nucleotide mutations (marked by m) and annotation as shown in Table 1 and GenBank access no. KF322026 and KF800937. This draw was done by BLAST [9] and Ring Image Generator [32]. Purple line represents skew(-); green for skew(+); black for GC%; grey for K1E; pink for SP6; blur red for ϕA318; cyan for ϕAs51; red for specific genes. (C) Mauve was used to efficiently construct multiple genome alignments, which provides a basis for research into comparative genomics among phages ϕA318 to its related phage K1E.