Figure 4

Analysis of clotting factor C like genes in insects. (A) Schematic representation of clotting factor C-like proteins from insect species. Red bars, green hexagons, orange circle and blue oblongs indicate the putative signal peptide sequence, LDLa domain, CCP domain and serine protease domain with the catalytic triad, respectively. The size bar indicates the amino acid residues of the deduced proteins. (B) Phylogenetic analysis of insect clotting factor C like genes. The phylogenetic tree was constructed based on the deduced amino acid sequences of clotting factor C like genes by maximum likelihood, using the program Mega 5.05 (http://www.megasoftware.net/). The Jones-Taylor-Thornton (JTT) model for amino acid substitution was used, while a test of phylogeny was carried out using the bootstrap analysis with 1000 replications. The GenBank accession numbers for the sequences are as follows: Nilaparvata lugens (KJ512060); Camponotus floridanus (EFN71201); Acyrthosiphon pisum (XP_001944706); Apis mellifera (XP_001120594); Bombus terrestris (XP_003403297); Danaus plexippus (EHJ70705); Bombyx mori (AFK93534); Culex quinquefasciatus (XP_001864236); Tribolium castaneum (XP_967486); Aedes aegypti (XP_001655952); Manduca sexta (AAR29602); Megachile rotundata (XP_003707203).