Figure 3

Organisation of the murine CrAT promoter. (A) Presentation of the 5′ flanking sequence of the murine CrAT gene. Consensus binding sites are underlined. (B) Schematic structure of the murine CrAT promoter with putative binding sites. mCrAT-1 represents the construct for luciferase-assays ranging from −342 bp to +15 bp and mCrAT-2 from −763 to −328 bp. The artificial promoter construct mCRAT-1 contained the RXR-box, two Sp1 elements and one CACbP region in front of the luc-gene of the pGL2-basic luciferase reporter vector. The promoter construct mCRAT-2 contained three YY-1 sites, two GR1 elements and a HNFβ box in front of the reporter luc-gene.