Figure 5

Extracellular parasite gene expression profiles are distinct from those of intracellular parasites. A: GSEA comparison of Extracellular and Intracellular Tachyzoites. Plotted normalized enrichment scores (NES) for tachyzoite/bradyzoite gene sets (top) and cell cycle gene sets (bottom). A positive NES indicates that the gene set is associated with extracellular tachyzoite parasites, negative NES indicates that the gene set is associated with intracellular tachyzoites. For tachyzoite/bradyzoite gene sets, stars indicate significant enrichment (FWER-adjusted p < 0.05). Cell cycle plots show only gene sets with significant enrichment. Relative to proliferating intracellular tachyzoites, extracellular tachyzoites are enriched in G1 genes and more “bradyzoite”-like. B: GSEA comparison of Extracellular Tachyzoites and in vitro Bradyzoites. Plotted normalized enrichment scores (NES) for tachyzoite/bradyzoite gene sets (top) and cell cycle gene sets (bottom). A positive NES indicates that the gene set is associated with extracellular tachyzoite parasites, negative NES indicates that the gene set is associated with in vitro bradyzoites. For tachyzoite/bradyzoite gene sets, NES are shown for gene sets with FDR <25% and stars indicate significant enrichment (FWER-adjusted p < 0.05). Cell cycle plots show only gene sets with significant enrichment. Relative to proliferating intracellular “bradyzoites”, extracellular tachyzoites are enriched in G1 genes and less “bradyzoite”-like.