Figure 5

Using a yellow-to-blue color scale greatly increases the range of visual discrimination. When white-to-black (A) and yellow-to-blue (B) color scales are compared against different backgrounds, it is apparent that the latter permits visual discrimination of greater variation, especially at the extremes where the white-to-black scale contrasts with the background. Another advantage of the yellow-to-blue color scale is that the middle grey is distinct from the hues at either extreme and can be used to indicate those bases whose occurrence coincides with random chance. When comparing consensus sequences rendered in the black, grey, and yellow-to-blue color scales (C), sequence variation within the HssRS DNA binding motif[11] is clearest when viewed using the yellow-to-blue scale.