Figure 4

Simplified model of predicted miRNA targets related to Calpain1, JNK/p38 MAPK, MAPK/ERK and Akt/mTOR pathways. Igf functions are mediated by the Igf-I receptor (Igf1R) through activation of two major intracellular signalling pathways: MAPK and mTOR. mTOR and p38 MAPK are required for myogenic differentiation and p38 MAPK also inhibits proliferation. miR-130c was predicted to regulate EIF4E and MAPK9, miR-17a-5p may bind to the 3’UTRs of Sestrin3, MAPK3 and MAPK13, miR-181a-5p could target DDIT4 and MAPK3, miR-181a-3p may regulate Calpain1 and miR-206-3p was predicted to target Sestrin1. In Senegalese sole, miR-17a, miR-181-5p and miR-206 levels were higher at 21°C than 15°C during somitogenesis, whereas miR-181a-3p was down-regulated at 21°C compared to 15°C. Arrows, circles and horizontal lines indicate activation, negligible effect and inhibition, respectively.