Figure 1

Methyl-β-cyclodextrin enhances muscle differentiation. Chick myogenic cells were grown for 24 hours, treated with 2 mM of methyl-β-cyclodextrin (MbCD) for 30 minutes and grown for the next 24 hours. Untreated (A) and MbCD-treated cells (B) were fixed and double-stained with an anti-sarcomeric α-actinin antibody (green) and the nuclear dye DAPI (blue). Merged images are shown in A and B. Note the α-actinin distribution in Z-lines along sarcomeres in both untreated and MbCD-treated muscle cells (arrows in A and B). Scale bar in B represents 10 μm.