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Table 1 Summary of the number of reads at each processing step

From: A comprehensive analysis of piRNAs from adult human testis and their relationship with genes and mobile elements

Step/Sample

S9217

S7964

S7963

Total

 

Total

Unique

Total

Unique

Total

Unique

Total

Unique

Raw reads

6.85

1.89

25.30

7.41

22.91

7.57

55.05

15.07

Step 1

6.19

1.77

22.79

7.04

21.87

7.49

50.85

14.51

Step 2

3.84

1.57

14.13

6.39

13.05

6.72

31.02

13.31

Step 3

2.62

1.26

12.68

5.76

11.86

6.19

27.17

11.91

Step 4

1.47

0.82

8.83

3.55

7.13

3.22

17.44

6.66

  1. Step 1. Remove 3’adapter sequence and remaining reads of size between 5 ~ 45 nt.
  2. Step 2. Remove known small RNAs.
  3. Step 3. Remove unmapped reads to the reference genome (hg19).
  4. Step 4. Select reads of size between 26 ~ 31 nt.
  5. Number of reads is in millions.
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BMC Genomics

ISSN: 1471-2164

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