Genome sequencing of four Aureobasidium pullulans varieties: biotechnological potential, stress tolerance, and description of new species

Table 5 Numbers of specific types of alkali-metal cation transporters of the four Aureobasidium pullulans varieties

Cell location	Transporter type	Substrate specificity/main function	Sc homologue	Number per A. pullulans variety*
				ApP	ApS	ApN	ApM
Plasma membrane	Channel	K⁺ efflux	Tok1	2	2	2	2
	Uniporter	K⁺ uptake	Trk1,2	3	2 (+1)	2	2
	Antiporter with H⁺	K⁺ uptake (Hak)	/	1	1	1	1
	P-type ATPase	K⁺ uptake (Acu)	/	2	2	2	2
	P-type ATPase	Na⁺ (and Li⁺) efflux	Ena1,2,5	1	2	2	2
	Antiporter with H⁺	Na⁺ (and K⁺) efflux	Nha1	3	3	3	3
	Symporter	Na⁺/P_i cotransporter	Pho89	3	3	1	2
	P-type ATPase	H⁺ export	Pma1,2	1	1	1	1
Golgi apparatus	Antiporter	K⁺/H⁺ exchange	Kha1	1	1	1	1
Golgi apparatus	P-type ATPase	Ca²⁺ and Mn²⁺ transport into Golgi apparatus	Pmr1	2	2	2	2
Late endosomes	Antiporter	Na + (and K+) efflux	Nhx1	1	1	1	1
Vacuole	Antiporter	Na⁺, Ca²⁺/H⁺ exchange	Vnx1/Vcx1	1 (4)	1 (4)	1 (4)	1 (4)
	V-type ATPase (4 subunits)	H⁺ in vacuole	Vma1	1	1	1	1
	P-type ATPase	Depleting cytosol of Ca²⁺ ions	Pmc1	1	1	1	1
Mitochondria	Antiporter	K⁺/H⁺ exchange	Mrs7/Mdm38	1	1	1	1

*ApP, A. pullulans var. pullulans; ApS, A. pullulans var. subglaciale; ApN, A. pullulans var. namibiae; ApM, A. pullulans var. melanogenum; Sc, S. cerevisiae.

ISSN: 1471-2164