Figure 1

Outline of fermentation study. Schematic of the fermentation screen (micro-fermentations; 0.6 mL and laboratory-scale; 100 mL fermentations) and number of gene deletion mutants identified at each stage as having protracted fermentation. Final evaluation using 100 mL fermentations identified 72 candidate genes. In silico analysis of fermentation relevant data (SGD_FRYG and LIT_FRYG databases) and GO analysis of genes functionally related to 72 candidate genes. 21 additional gene deletants were identified as resulting in protracted fermentation. Together, the 93 genes are referred to as Fermentation Essential Genes or the ‘Fermentome’. FD denotes fermentation duration.