Transcriptome analysis of callus from Picea balfouriana

BMC Genomics

Table 4 Statistical output from the BestKeeper analysis for the Picea balfouriana candidate reference genes

	WS00912.B21_N13	WS0109_C05	18SrRNA	Tubulin
N	6	6	6	6
G Mean [Ct]	28.03	27.57	13.12	24.37
A Mean [Ct]	28.06	27.58	13.13	24.4
Min [Ct]	26.21	26.38	12.33	22.62
Max [Ct]	29.78	28.79	13.81	25.68
SD [±Ct]	0.85	0.58	0.56	1.06
CV [% Ct]	3.02	2.09	4.26	4.35
Coeff. of corr. [r]	0.85	0.947	-0.014	0.605
P-value	0.032	0.004*	0.978	0.203

N, number of samples; G Mean [Ct], geometric mean of the Ct; A Mean [Ct], arithmetic mean of the Ct; Min [Ct] and Max [Ct], extreme values of the Ct; SD [±Ct], standard deviation of the Ct; CV [% Ct], coefficient of variance expressed as percentage on the Ct level. Coeff. of corr. [r], coefficient of correlation. *Indicates the best correlation between the control genes and the BestKeeper index.

ISSN: 1471-2164