Figure 3
From: Evaluating bias-reducing protocols for RNA sequencing library preparation
Over-representation in sequencing libraries. Sequencing libraries were generated from the partially degenerate RNA pool using either the standard protocol (standard), the CircLig protocol with trRnl2 K227Q (rnl2) or the CircLig protocol with Mth K97A (mth). The abundance (read density) of each unique sequence within the degenerate region was calculated as a ratio of the total read data (reads per million sequenced; RPM). The density of the 1000 most abundant sequences are presented for each library. The theoretical is X ~ Binominal(3x106, 1/410).