Figure 3

Results obtained of the 100 isolates by various methods. Samples were taken from 100 individual patients, all diagnosed with pulmonary TB and producing AFB positive sputum smear; MLPA, DST, GenoTypeMTBDRplus, spoligotyping and MIRU-VNTR was performed on all 100 isolates. DST for first line drugs was performed on all 100 isolates whereas DST for second line drugs was performed on drug-resistant TB isolates only. DST results were not available for four isolates due to contamination of the respective cultures; No information was obtained from three isolates tested by GenoTypeMTBDRplus; Spoligotypes were not obtained for one isolate. Unknown spoligotypes were obtained for 17 isolates and the spoligotypes of five isolates were not reported in the SITVITWEB database. MIRU-VNTR types were not obtained for 13 isolates. Multiple copy numbers in one or more loci were revealed in two isolates; Genotypic information of 45 markers screened per isolate was obtained for all 100 isolates by MLPA. For 85 isolates, lineage types could be assigned on the basis of consistent lineage marker profiles and in 14 isolates after expert review. For one isolate the lineage type profile was not interpretable. For 46 isolates molecular drug resistance was identified by MLPA of which 11 isolates had intermediate signals for at least one drug resistance conferring marker.