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Table 2 Correlation between drug resistance identified by MLPA and GenoTypeMTBDR plus

From: Optimizing multiplex SNP-based data analysis for genotyping of Mycobacterium tuberculosis isolates

  Drug resistance by GenoTypeMTBDRplus
Drug resistance by MLPA rpoBWT rpoBMUT3 rpoBMUT othera katGWT katGMUT1 katGMUT otherb inhAWT inhAMUT1 inhAMUT1 and katGMUT1 No result
no rpoB mutation 76 0 3        2
intermediate rpoB 6 1 0        1
rpoB-S531L (rpoBMUT3) 0 11 0        0
rpoB-S522L 1 0 0        0
no katG mutation     74 0 0     1
katG-S315T (katGMUT1)     0 14 1     1
intermediate katG     1 0 0     0
no inhA mutation        64 0 0 2
inhA-15 C/T (inhAMUT1)        6 2 0 0
inhAMUT1 and katGMUT1        0 0 5 1
intermediate inhA        5 0 0 0
Total isolates (GenoTypeMTBDRplus) 82 12 3 75 14 1 75 2 5 3
  1. Mutations obtained by MLPA are indicated for the respective genetic marker investigated with the GenoTypeMTBDRplus assay; isolates for which no results were obtained by GenoTypeMTBDRplus MLPA results for all genetic markers are indicated; an intermediate result indicates the presence of an intermediate signal and thus no presence or absence for the respective genetic marker; a = two isolates did not show a positive band with the rpoBWT8 probe but no mutation probe was positive; one isolate did not show a positive band for the rpoBWT3 and rpoBWT4. b = one isolate did not show a positive band with the katGWT1 probe but no mutation probe was identified.