Optimizing multiplex SNP-based data analysis for genotyping of Mycobacterium tuberculosis isolates

BMC Genomics

Table 2 Correlation between drug resistance identified by MLPA and GenoTypeMTBDR plus

	Drug resistance by GenoTypeMTBDRplus
Drug resistance by MLPA	rpoBWT	rpoBMUT3	rpoBMUT other^a	katGWT	katGMUT1	katGMUT other^b	inhAWT	inhAMUT1	inhAMUT1 and katGMUT1	No result
no rpoB mutation	76	0	3							2
intermediate rpoB	6	1	0							1
rpoB-S531L (rpoBMUT3)	0	11	0							0
rpoB-S522L	1	0	0							0
no katG mutation				74	0	0				1
katG-S315T (katGMUT1)				0	14	1				1
intermediate katG				1	0	0				0
no inhA mutation							64	0	0	2
inhA-15 C/T (inhAMUT1)							6	2	0	0
inhAMUT1 and katGMUT1							0	0	5	1
intermediate inhA							5	0	0	0
Total isolates (GenoTypeMTBDRplus)	82	12	3	75	14	1	75	2	5	3

Mutations obtained by MLPA are indicated for the respective genetic marker investigated with the GenoTypeMTBDRplus assay; isolates for which no results were obtained by GenoTypeMTBDRplus MLPA results for all genetic markers are indicated; an intermediate result indicates the presence of an intermediate signal and thus no presence or absence for the respective genetic marker; a = two isolates did not show a positive band with the rpoBWT8 probe but no mutation probe was positive; one isolate did not show a positive band for the rpoBWT3 and rpoBWT4. b = one isolate did not show a positive band with the katGWT1 probe but no mutation probe was identified.

ISSN: 1471-2164