Figure 4

Glycolysis and the triacylglycerol biosynthetic pathway in Cp0710. (A) Change of enzyme expression in glycolysis under heterotrophic growth conditions. The name of each enzyme is in red and the corresponding protein ID in Cp0710 is shown in blue. Values in brackets indicate changes in RNA (left) and protein (right) levels, respectively. U: only identified in heterotrophic approach (upregulation). D: only identified in autotrophic approach (downregulation). N: not detected. (B) Change in enzyme expression in the triacylglycerol biosynthetic pathway under heterotrophic growth conditions. The color code is the same as in (A). Abbreviations of enzymes are as follows: HT, H⁺/hexose co-transporter; HK, hexokinase; GPI, glucose-6-phosphate isomerase; PFK, 6-phosphofructokinase; ALDO, fructose-bisphosphate aldolase; TPI, triose phosphate isomerase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PGK, phosphoglycerate kinase; PGAM, phosphoglycerate mutase; ENO, enolase; PK, pyruvate kinase; Accase, acetyl-CoA carboxylase; MCAT, acyl-carrier protein (ACP) S-malonyltransferase; KAS, 3-oxoacyl-ACP synthase II; KAR, 3-oxoacyl-ACP reductase; HD, 3-hydroxyacyl-ACP dehydratase; ENR, enoyl-ACP reductase I; FATA, fatty acyl-ACP thioesterase A; ACS, long-chain acyl-CoA synthetase; G3PDH, glycerol-3-phosphate dehydrogenase; GPAT, glycerol-3-phosphate O-acyltransferase 3/4; LPAAT, lysophosphatidate acyltransferase; PAP, phosphatidate phosphatase; DGAT, diacylglycerol O-acyltransferase 1; MLDP, major lipid droplet protein.