Figure 1
Phylogenetic tree of putative H. armigera ORs with other insect ORs. (A) The phylogenetic tree based on OR sequences from H. armigera, B. mori, D. plexippus and H. melpomene. (B) The phylogenetic tree based on PR sequences from 10 noctuid moths. The tree was constructed using PhyML under the JTT model of substitution with NNI topology search, based on an amino acid alignment by ClustalW2. Branch support was estimated using an approximate likelihood ratio test (Chi2) (circles: > 0.98). HarmORs are in bold and newly identified candidate HarmORs are highlighted with red letters. In Figure 1B, these PR genes with known ligands are labeled with red triangles, including H. armigera (this study, [18]), H. virescens [19, 20], Mythimna separate [21], S. exigua [22], and S. littoralis [23]. Bmor: B. mori; Dple: D. plexippus; Harm: H. armigera; Hmel: H. melpomene; Hvir: H. virescens; Hsub: H. subflexa; Hzea: H. zea; Msep: Mythimna separate; Sexi: S. exigua; Slit: S. littoralis; Slitu: S. litura; Snon: S. nonagrioides; Tni: Trichoplusia ni.