Figure 4

Gene expression profiles of four P. sulphurascens genes down-regulated in infected Douglas-fir seedling roots. By qRT-PCR with ubiquitin conjugating enzyme E2 (Ps uceE2) as an internal control gene, relative quantities of the four genes were measured in vegetative mycelia and infected DF seedling roots collected at eight time points from 12 hours to 7 days post-inoculation. This experiment was performed with three biological replicates with triplicate technical replicates. Error bars represent standard deviations. Met = metalloprotease, Mn-SOD = manganese superoxide dismutase.