Figure 5

Itga11 knockdown decreased mRNA and protein amount. A. Relative quantities of Itga4 (soft grey) and Itga11 (white) mRNA for treated cultures, or untreated cultures (black) in MSC. For treated cultures with negative control shRNA (dark grey), both Itga11 and Itga4 mRNA were quantified but to simplify, only the relative quantity of Itga11 is shown. B. Detection of ITGA11 by western blot during myogenesis. Proteins were extracted from MSC culture, before (0 h) and during differentiation (24 h, 48 h and 72 h) and the quantities of ITGA11 were revealed by western blot using Actin as control.