Chst5 knock-down decreased mRNA and protein amount. A. Relative quantities of Chst5 mRNA for treated cultures (white), or untreated cultures (black) in MSC and for treated cultures with negative control shRNA (dark grey), Chst5 mRNA were quantified by RT-PCR. B. Western blot detection of CHST5 during myogenesis. Proteins were extracted from MSC culture, before (0 h) and during differentiation (24 h, 48 h and 72 h) and the relative quantities of Chst5 were revealed by western blot using Actin as control.