Identification of a novel CD46 splice variant. (A) Genomic structure of the bovine CD46 gene. CD46 gene consists of 12 exons, comprising 1257 bp of coding sequence. The positions of the primers (CD46F, CD46R) used in the CD46 cloning experiment are indicated by black arrows. (B) The splicing pattern of the CD46-TV splice variant. The CD46-TV (GenBank accession number: KM114056) transcript retains a 48 bp sequence from intron 8. The F1 and R1 primers are located within the 48 bp retained intron sequence and exon 9, respectively. The position of the A nucleotide in the start codon (ATG) is defined as +1.