Figure 4

Putative chimeric RNAs in M. pneumoniae and P. aeruginosa . A) Circos plot representing chimeric RNAs in the genome of M. pneumoniae. Rectangles represent a zoom of the genomic region indicated with a black arrow. These regions correspond to the two parts that compound the different chimeric RNAs described in Additional file 2: Table S2 (a = region of first gene and b = region of second gene). Red arrows in the rectangles indicate genes and lines in Circos plot indicate connections between genomic regions. B) Secondary structure of M. pneumoniae RNA species. Upper part of the panel shows the predicted secondary structure of a putative chimeric RNA of M. pneumoniae (MpnCh2) obtained by CLC Workbench program. Lower panel shows an example of the secondary structure for the MpnCh1, a circular RNA of M. pneumoniae. In both representations the junction is indicated with a red arrow and second part of the chimeric RNA is shadowed in blue. Complementary regions are connected with black lines. C) Circular RNA of P. aeruginosa. Circular RNA corresponding to the results described in Additional file 2: Table S3. Red arrow indicates 23S rRNA gene, blue and black lines indicate the genome positions of the first and second regions of the circular molecule, respectively. Circular graph is a schematic representation of the obtained molecule. D) Secondary structure of the 23s rRNA chimera found in P. aeruginosa. The junction is indicated with a red arrow and second part of the chimeric RNA is shadowed in blue. Complementary regions are connected with black lines.