Figure 4

Localization of HOXC11 protein across the tissues of buffalo. HOXC11 was detected in the nuclei by IHC using rabbit anti-HOXC11 antibodies and goat anti-rabbit HRP antibodies. Nuclei were counterstained by hematoxylin. Images were taken in bright field (DIC) at 60× magnification. (A-H) indicate brain, heart, spleen, testis, liver, ovary, kidney and lung, respectively. I) Western blot analysis of HOXC11 protein in different somatic and gonadal tissues of buffalo using rabbit anti-HOXC11 IgG. The differential expression of HOXC11 protein across the tissues was normalized against β-actin. J) The expression (in folds) of HOXC11 protein was calculated using Image J software. The standard bars indicate the reproducibility of the experiment.