Table 2 Functional prediction of hypothetical proteins identified in Bradyrhizobium diazoefficiens CPAC 7 whole protein extract based on protein sequences, conserved domains and motifs, protein-protein interactions and cellular locations
Spot ID | Hypothetical protein | NCBI ID | Cellular location | *T/**E pI | *T/**E MW | Predicted function |
|---|---|---|---|---|---|---|
Metabolism | ||||||
Amino acid transport and metabolism | ||||||
25 | Blr3064 | gi|27378175 | Cytoplasmic | 5.64/6.00 | 50837/49000 | Succinyl-diaminopimelate desuccinylase (COG/NCBI); Peptidase family M20 - dimerisation domain (Pfam); GO: Hydrolase activity (InterPro); Lysine biosynthesis EC:3.5.1.18 (KEGG). |
30 | Blr5678 | gi|27380789 | Periplasmic | 5.84/5.11 | 33744/27000 | L-aminopeptidase/D-esterase (COG); Peptidase family S58 (Pfan); GO:arginine biosynthetic process; DmpA/ArgJ- Like domains (InterPro). |
Coenzyme transport and metabolism | ||||||
36 | Blr3798 | gi|27378909 | Cytoplasmic | 5.66/6.13 | 27452/29000 | Demethylmenaquinone methyltransferase (COG); Methyltransf_6 (Pfam);GO: methyltransferase activity (UniProtKB); Ribonuclease E inhibitor RraA domain (InterPro). |
37 | Bll4565 | gi|27379676 | Cytoplasmic | 5.3/5.59 | 24885/28000 | Demethylmenaquinone methyltransferase (COG); Methyltransf_6 (Pfam);GO: methyltransferase activity (UniProtKB); Ribonuclease E inhibitor RraA domain (InterPro). |
Information storage and processing | ||||||
Transcription | ||||||
72 | Bll4752 | gi|27379863 | Cytoplasmic | 4.41/4.43 | 27960/32000 | Predicted transcriptional regulator containing the HTH domain (COG); Putative transcriptional regulators (Ypuh-like)(Pfam); Winged helix-turn-helix DNA-binding domain (InterPro). |
Cellular processes and signaling | ||||||
Posttranslational modification, protein turnover, chaperones | ||||||
83 | BJ6T_08050*** | gi|354953419 | Cytoplasmic | 4.46/4.43 | 20515/28000 | Thioredoxin-like proteins and domains(COG); Scaffold protein Nfu/NifU N terminal (Pfam); GO: Iron-sulfur cluster binding (InterPro). |
Signal transduction mechanisms | ||||||
87 | Blr2761 | gi|27377872 | Cytoplasmic | 5.465.70 | 29257/33000 | Universal stress protein UspA (COG); Universal stress protein family (Pfam); GO: response to stress (InterPro). |
Poorly characterized | ||||||
General function prediction only | ||||||
92 | Bll5663 | gi|27380774 | Cytoplasmic | 4.92/5.08 | 33547/34000 | MoxR-like ATPases (COG); ATPases Associated with diverse cellular Activities – AAA proteins (Pfam); GO: ATPase activity (InterProt). |
Function unknown | ||||||
94 | Blr5067 | gi|27380178 | Cytoplasmic | 5.25/5.29 | 24247/29000 | Uncharacterized ACR (COG); putative metal binding site - region_name = "LabA (NCBI); NYN domain (Pfam). |
95 | Blr5067 | gi|27380178 | Cytoplasmic | 5.93/5.96 | 16852/28000 | Uncharacterized ACR (COG); putative metal binding site - region_name = "LabA (NCBI); NYN domain (Pfam). |
NO related COG | ||||||
99 | Bll0565 | gi|27375676 | Periplasmic | 4.98/5.04 | 41554/57000 | No related data |
101 | Blr7534 | gi|27382645 | Periplasmic | 5.86/4.99 | 49518/45000 | No related data |
107 | Bll5131 | gi|27380242 | Extracellular | 7.68/5.80 | 34214/32000 | Protein of unknown function DUF (Pfam/InterPro). |
108 | Blr2961 | gi|27378072 | Cytoplasmic | 4.96/5.06 | 25510/29000 | GO: catalytic activity/Metabolic process (InterPro); Protein of unknown function (Pfam). |
109 | Bll5307 | gi|27380418 | Periplasmic | 5.55/5.70 | 14177/25000 | No related data |
110 | Blr2191 | gi|27377302 | Cytoplasmic | 6.58/6.12 | 25491/27000 | Uncharacterized protein conserved in bacteria (DUF2328) (Pfam). KO: chpT histidine phosphotransferase (KEGG); Two-component system/His Kinase A (phospho-acceptor) domain (IMG). |
112 | Bll7551 | gi|27382662 | Cytoplasmic | 5.95/5.92 | 27565/26000 | No related data |
113 | Blr0227 | gi|27375338 | Periplasmic | 5.17/5.29 | 22619/25000 | PHB accumulation regulatory domain (Pfam);GO: Regulation of transcription/Transcription repressor activity (NCBI/InterPro). |
114 | Blr0227 | gi|27375338 | Periplasmic | 5.17/6.40 | 22619/24000 | PHB accumulation regulatory domain (Pfam);GO: Regulation of transcription/Transcription repressor activity (NCBI/InterPro). |
115 | Blr7436 | gi|27382547 | Cytoplasmic | 4.82/4.90 | 15290/18000 | No related data |